产品目录

分子量：Approximately 18.2kDa,a single non-glycosylated polypeptide chain containing158 amino acids.
储存条件：冷冻（-20℃）

概述

MANF is a secreted neurotrophic factor that is expressed in brain,neuronal and certain non-neuronal tissues.It has been shown to promote survival,growth and function of dopamine specific neurons.MANF and its structural homolog CDNF,each contain an N-terminal saposin-like lipid binding domain,and a carboxyl-terminal domain,which is not homologous to previously characterized protein structures.MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease.Mature human MANF is 99%,98%and 96%a.a.identical to mature rat,mouse and bovine MANF respectively.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞95%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20μg/ml,corresponding to a specific activity of>50 IU/mg.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4.
内毒素	Less than 1 EU/μg of rHuMANF as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL

相关搜索：重组人MANF，MANF, Human